High performance liquid chromatography (HPLC) is the most commonly used method for analyzing substances. This
book brings readers completely up-to-date on the latest HPLC methods. It covers principles, instrumentation, applications,
and procedures and features new and expanded chapters on theory, sample preparation techniques, computer-assisted
method development, and biochemical/chiral applications.
Table of Contents
Getting Started.
Basics of Separation.
Detection Sensitivity and Selectivity.
Sample Preparation.
The Column.
Non-Ionic Samples: Reversed- and Normal-Phase HPLC.
Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.
Gradient Elution.
Systematic Approach to the Reversed-Phase Separation of Regular Samples.
Computer-Assisted Method Development.
Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.
